NCI-H520-EGFP cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

NCI-H520-EGFP Order

Catalog#

YC-C017-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium-D

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

NCI-H520-EGFP

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Accession

CVCL_C8X0(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NCI-H520			STR Info (Cell bank) Cell Line:NCI-H520 [H520]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	13		12	13
D13S317	10	11		10	11
D7S820	8	12		8	12
D16S539	8	13		8	13
VWA	18	19		18	19
TH01	10	10		10	10
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	10		10	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the NCI-H520 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NCI-H520-EGFP cell line that can maintain long-term, stable and high expression.